Figure 1
From: A high-throughput pipeline for the design of real-time PCR signatures
Components of a real-time PCR signature. A real-time PCR signature consists of a forward primer, a probe, and a reverse primer. The lengths of these three components, the distances between them, and the total amplicon length can vary greatly depending on the real-time PCR technology. The values shown in the figure are typical for the TaqMan® real-time PCR assays.