Figure 2
From: A high-throughput pipeline for the design of real-time PCR signatures
Overview of the TOPSI pipeline. The pre-processing stage of TOPSI obtains consensus sequence segments that are common to all input genomes. The actual signature design process, including comparison with non-target genomes, is performed in the three stages of the core TOPSI pipeline. The post-processing module assembles individual unique primers and probes into PCR signatures.