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Table 2 Specificity thresholds used in TOPSI runs for S. aureus

From: A high-throughput pipeline for the design of real-time PCR signatures

Specificity parameter Strict threshold Relaxed threshold
M0 - longest stretch of contiguous matches with a non-target that has no mismatches 18 18
M1 - longest stretch of contiguous matches with a non-target that has at most one mismatch 20 40
M2 - longest stretch of contiguous matches with a non-target that has at most two mismatches 22 40
M3 - longest stretch of contiguous matches with a non-target that has at most three mismatches 24 40
Maximum overall identity with a non-target sequence 90% 90%