Figure 1

Importance ranking of T _m , the minimal free energy (mfe) of probe structure, and probe length. All probes: Importance ranking of T _m , the minimal free energy (mfe) of probe structure, and probe length. Here, 'Tm' stands for the melting temperature, 'mfe' labels the minimal free energy of probe secondary structure and 'len' the probe length. Probe lengths ranged from 45 to 74 nt. Error bars are from 100 random bootstrap samples of 200,000 probes each.