Comparison of datasets |
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Parameter
| Pozhitkov et al. [26] | Wei et al. [23] |
probe | DNA | DNA |
target | rRNA | cDNA |
probe length | 25-mers | 45- to 75-mers |
resolution | 1 nt | 22 nt |
number of probes | 7, 519a | 21, 813b |
number of targets | 9a | 2, 472b |
target concentration | 375 ng | Unknown |
- a We analysed the probes perfectly matching the respective separately hybridized individual targets, ensuring no crosshybridization effects.
- b We focused on probes targeting clearly expressed transcripts and showing no cross-hybridization in our analysis.
- Caption: This table summarizes the characteristic differences between the two datasets studied. The dataset of Pozhitkov et al. [26] comprises tiling probes targeting 9 rRNA fragments. The dataset of Wei et al. [23] consists of tiling probes designed for high-resolution interrogation of the entire human genome. The row 'probes' shows the nucleic acid type of probes, row 'target' the nucleic acid type of targets.