CM Engine acceptable image formats. Red dots in the Figure indicate position A1. Red dots are for illustrative purpose only and should not appear on images processed by CM Engine. Images are assumed to be in this orientation by the CM Engine. Note: images are not shown at 100% scale. Full size example images may found in Additional File 2 or may be downloaded from the Rothstein Lab website: http://www.rothsteinlab.com/tools/screen_mill/cm_engine.