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Figure 5 | BMC Bioinformatics

Figure 5

From: Extended morphological processing: a practical method for automatic spot detection of biological markers from microscopic images

Figure 5

Experimental results using synthetic images. (a) Example of source image with an 8-bit gray-scale. (b) 2-D Gaussian distributions as spot models. Two spot models with different intensity levels were used in this experiment. The actual image of the spot models (cropped from the white dotted rectangular region of (a)) are shown on the left, and the intensity profiles on the right. (c) Example results of spot detection using synthetic noise images with a uniform (type-A) and a gradient (type-B) background. The performance of the proposed method was compared with that of conventional top-hat (TH) and h-dome (HD) transformation. (d) The recall, precision, and F-measure rates of this experiment. (e) The performance of our method when synthetic images with various noise levels are used.

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