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Table 1 Adjusted Relative Expression.

From: Efficiency clustering for low-density microarrays and its application to QPCR

Adjusted Relative Expression
Primer Cluster efficiency C T Adjusted C T : Fold difference:
orfK6 1.72 24.15 18.85 39.28
orf7-1632F 1.81 23.71 20.39 10.00
orf21-1578F 1.86 23.85 21.32 5.78
orf4-1511F 1.89 23.29 21.38 3.75
orf9-708F 1.94 24.24 23.16 2.12
orf2-137F 2.03 26.13 26.76 0.64
tac29-5F 2.14 25.78 28.34 0.17
  1. Raw and adjusted C T values are shown for seven primer pairs, belonging to different efficiency clusters. Fold difference is calcuated as the estimated concentration under perfect efficiency, divided by concentration under the adjusted efficiency. The assumption of perfect efficiency can lead to multiple fold inaccuracies in relative expression.