Increased sensitivity of the proposed method: Example for the comparison between t-test and z-test (our approach) for the case of 1000 simulated "genes", each with three repeats. 500 out of the 1000 "genes" were truly differentially expressed, (see text for full explanation). For each FDR level the true positive rate (TPR) and the false positive rate (FPR) were calculated based on the ground truth. (a) TPR versus FDR level, (b) FPR versus FDR level. The blue line indicates results of t-test and the red line indicates z-test.