Application for time course experiment and comparison to other methods: (a) Estimated standard deviation vs. intensity lines derived from two repeats of data from t = 16 hrs and from t = 24 hrs after LPS stimulus. (b) Scatter plot of mean intensities measured at t = 16 hrs vs. t = 24 hrs. Blue dots denote genes that were found as non-significantly differentiating (by our method); magenta - differentially expressed genes, that passed at FDR of 5%; green line - 1 SD. (c)-(j) Venn diagrams of the three groups of genes identified as differentially expressed at 5% FDR in each comparison of consecutive time points by: 2 fold (yellow), SAM (red), and our method (blue), (data used: GSE17721). Note that t-test is not part of the comparison since significantly differentiating genes were found at this FDR only in the 2 hr vs. 4 hr comparison.