Figure 1

Experimentally-derived peak regions subdivided into individual sites of signal enrichment. A) Region of Oct4 binding in the Abca4 gene locus, subdivided into six individual loci by the PeakSplitter utility. The canonical binding sequences of Oct4, Nanog and Sox2 are concentrated in the center peaks, while potentially novel motifs or sites of associated co-factor binding may be present in the adjacent three. Peak splitting is equally applicable to the broader regions of enrichment generated from chromatin modification experiments, where signal peaks are more variable in size and shape. Depicted is a region of H3K4me3 methylation on chromosome 16 [29], subdivided into four discrete modification sites.