Figure 2From: Coherent pipeline for biomarker discovery using mass spectrometry and bioinformaticsRefinement of peptides. Unique Peptides (UP) were identified by selecting the peptides that had an exact hit in BLAST against the NCBI non-redundant database. These peptides were refined by selecting the consensus of peptides in all biological triplicates (Rep ∩ 1 ∩ 2 ∩ 3) followed by selecting the peptides that are not present in non-Clostridium botulinum species (Non-CBOT). The final phase selects only those peptides that are present in all six C. botulinum strains (All strains). We have labelled these peptides as 'Biomarkers'.Back to article page