Figure 1

Illustration of bivariate manual gating by a flow cytometry expert: Dr John Zaunders of the Centre for Immunology, Sydney, Australia. The gates correspond to the red-coloured shapes. The flow cytometry data correspond to a study on graft-versus-host disease (source: Brinkman et al. [4]). The panels correspond to day number with respect to blood and marrow transplant of a particular patient. The vertical axis is sinh^-1(side-scatter), whilst the horizontal axis is sinh^-1(fluorescence) for the second channel. Since the data are large flowViz defaults to displaying the data as smoothed scatterplots, based on bivariate kernel density estimation.