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Figure 3 | BMC Bioinformatics

Figure 3

From: SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data

Figure 3

Distribution of longest read segments passing a user-defined quality threshold (here, P = 0.05, or equivalently, Phred quality score Q ≈ 13, or a base call error rate of 1-in-20). Note that reads in this dataset would be trimmed on average to ~25 nucleotides (i.e., only approximately one-third of the initial 75 nucleotide read length). The defects evident in this dataset (see text for details) are atypical of Illumina sequencing; this dataset was chosen specifically to illustrate the capabilities of SolexaQA.

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