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Figure 7 | BMC Bioinformatics

Figure 7

From: Misty Mountain clustering: application to fast unsupervised flow cytometry gating

Figure 7

Flow chart of the ANALYZE routine of the Misty Mountain program. ISZM - the largest label of the aggregates in a cross section. (For simplicity this flowchart assumes that ISZM is also the number of aggregates. In reality ISZM is frequently larger than the number of aggregates.) ICLU - code number of a peak. ILAB - label of an aggregate. IP - counter of peaks belonging to the same aggregate. CP - characteristic position of a peak. IIPOINT1(ICLU) - characteristic position of the ICLU-th peak. IIPOINT2(ICLU) - label of the aggregate at the ICLU-th peak. IIPOINT3(ICLU) = T - the type of the ICLU-th peak: T = 1 - single peak, T = 0- merged small peak, T = -1 - merged major peak. The values of the IIPOINT2 and IIPOINT3 vector elements are updated at each level. IILEVEL1(ICLU) - level at the top of the ICLU-th peak. IILEVEL2(ICLU) - level of the saddle where the single ICLU-th peak coalesces with another peak. IHELP(ILAB) - number of characteristic peak positions falling into an aggregate labeled by ILAB. IHELP(ILAB,IP) - the code number of the peak belonging to the IP-th characteristic peak position in the aggregate labeled by ILAB. IFINAL(ICLU) = 0 - when ICLU-th peak is eliminated from the analysis. IFINAL(ICLU) = 1 - when the aggregate belonging to the ICLU-th peak is copied into NSTRF. ISZVEC(IP) = -1 - the IP-th peak has merged with other peak at a higher level. ISZVEC(IP) = 0- the IP-th peak was a small single peak at the previous level. ISZVEC(IP) = 1 - the IP-th peak was a major single peak at the previous level. INEW - number of single peaks merging with each other at the current level. IHIGH - number of major peaks from the INEW single peaks. Other notations are at the legends to Figure 6.

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