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Figure 5 | BMC Bioinformatics

Figure 5

From: Geoseq: a tool for dissecting deep-sequencing datasets

Figure 5

Choice of Tile-size. The number of hits for a particular miRNA changes with the tile-size used to query a sRNA-Seq dataset. The size of the tile is varied, while the start of the tile is held fixed at the beginning of the mature sequence of mmu-mir-24-1. At lower tile-sizes, the number of hits corresponds to fragments of the mature sequence that were sequenced. As the tile-size approaches the size of the miRNA, there is a drop-off in hits as there exist fewer reads that span the entire mature sequence. Finally, when the tile-size exceeds the length of the mature miRNA (22-bp), the number of hits drop to zero. The tile-size controls sensitivity and specificity, larger tile sizes increase specificity while smaller tile sizes increase sensitivity.

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