Figure 7
From: Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm
The SNP genotyping results of rs12449783 by (A) PCR-CTPP running in gel electrophoresis and (B) by TaqMan probe. (A) M and B indicate the 100 bp markers and blank, respectively. CC, AA, and AC indicate the control DNAs with known genotypes for CC, AA, and AC, respectively. The Pf 2/Pr 2(lanes 1, 5, and 9) and Pf 1/Pr 1(lanes 2, 6, and 10) primer pairs are designed to specifically amplify the A- and C-containing alleles for genotypes AA and AC vs. genotypes CC and AC, respectively. The Pf 1/Pr 2(lanes 3, 7, and 11) primer pair is designed to amplify the A- or C-containing alleles (CC, AA, and AC). For PCR-CTPP using four primers in one tube, it is marked with "Pf 1, Pf 2, Pr 1, Pr 2" for lanes 4, 8, and 12. The relative positions for Pf 1, Pf 2, Pr 1, and Pr 2are shown in Figure 1.