Overview of HOPE's process flow. The user submits a sequence and a mutation. HOPE will first collect information from a wide range of information sources. These sources include: WHAT IF for structural calculations on either the PDB file or a homology model that was build by YASARA, HSSP for conservation scores, DAS-servers for sequence-based predictions and Uniprot for sequence annotations. The data is stored in HOPE's information system. The data is combined with the known properties of the amino acids in a decision schedule. The result is a report shown on the HOPE website that will focus on the effect of the submitted mutation on the 3D-structure of the protein. The text and figures can be used in articles and publications.