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Table 4 Sensitivity and specificity of taxonomic binning at different taxonomic ranks using discarded dataset of simMC.

From: Reanalyze unassigned reads in Sanger based metagenomic data using conserved gene adjacency

   

Accuracy

Criteria

P

 

O

 

F

 

G

 

adjacency

E -value

hits

Sn

Sp

Sn

Sp

Sn

Sp

Sn

Sp

with

1e-2

50

31.8

97.2

28.3

90.8

27.6

84.8

24.1

88.8

  

150

34.9

94.5

30.8

86.2

30.0

79.3

25.3

82.0

  

250

36.8

93.3

31.7

84.0

30.7

77.0

25.9

79.0

  

350

37.9

92.7

32.0

83.0

30.7

75.8

25.7

77.6

 

1e-4

50

29.7

97.4

27.8

91.1

27.4

85.1

23.7

89.2

  

150

34.1

94.9

30.3

87.1

29.5

80.2

25.5

83.1

  

250

36.0

93.7

31.4

84.9

30.3

77.8

25.7

80.2

  

350

37.1

93.2

31.7

83.9

30.5

76.7

25.8

78.7

 

1e-6

50

29.1

97.5

27.3

91.2

26.8

85.1

23.4

89.2

  

150

33.1

94.9

29.7

87.1

28.8

80.2

24.7

83.1

  

250

35.0

93.7

30.8

84.9

29.8

77.8

25.5

80.2

  

350

36.2

93.2

31.3

83.9

30.2

76.7

25.8

78.7

without

1e-2

50

31.3

99.6

9.6

97.8

5.9

89.1

4.1

93.3

  

150

20.0

99.7

7.3

94.0

5.2

88.1

4.5

92.0

  

250

17.5

99.6

7.3

94.0

5.2

88.1

4.5

92.3

  

350

16.3

99.5

7.3

94.0

5.2

88.1

4.5

92.3

  1. Criteria considering gene adjacency and without considering gene adjacency were tested separately. P: phylum, O: order, F: family, G: genus. Sn and Sp denote sensitivity (%) and Specificity (%).