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Table 4 Sensitivity and specificity of taxonomic binning at different taxonomic ranks using discarded dataset of simMC.

From: Reanalyze unassigned reads in Sanger based metagenomic data using conserved gene adjacency

    Accuracy
Criteria P   O   F   G  
adjacency E -value hits Sn Sp Sn Sp Sn Sp Sn Sp
with 1e-2 50 31.8 97.2 28.3 90.8 27.6 84.8 24.1 88.8
   150 34.9 94.5 30.8 86.2 30.0 79.3 25.3 82.0
   250 36.8 93.3 31.7 84.0 30.7 77.0 25.9 79.0
   350 37.9 92.7 32.0 83.0 30.7 75.8 25.7 77.6
  1e-4 50 29.7 97.4 27.8 91.1 27.4 85.1 23.7 89.2
   150 34.1 94.9 30.3 87.1 29.5 80.2 25.5 83.1
   250 36.0 93.7 31.4 84.9 30.3 77.8 25.7 80.2
   350 37.1 93.2 31.7 83.9 30.5 76.7 25.8 78.7
  1e-6 50 29.1 97.5 27.3 91.2 26.8 85.1 23.4 89.2
   150 33.1 94.9 29.7 87.1 28.8 80.2 24.7 83.1
   250 35.0 93.7 30.8 84.9 29.8 77.8 25.5 80.2
   350 36.2 93.2 31.3 83.9 30.2 76.7 25.8 78.7
without 1e-2 50 31.3 99.6 9.6 97.8 5.9 89.1 4.1 93.3
   150 20.0 99.7 7.3 94.0 5.2 88.1 4.5 92.0
   250 17.5 99.6 7.3 94.0 5.2 88.1 4.5 92.3
   350 16.3 99.5 7.3 94.0 5.2 88.1 4.5 92.3
  1. Criteria considering gene adjacency and without considering gene adjacency were tested separately. P: phylum, O: order, F: family, G: genus. Sn and Sp denote sensitivity (%) and Specificity (%).