Work flow output. The figure shows the composite example image with the final result. As an easy reference, each sub-image is identified through its 'coordinates', given by the number of the row and the number of the column, to facilitate the output discussion. The areas shown in red are the affected ones; in case of unaffected areas they have been bordered in green. The gray areas represent non interesting zones, most of the time containing only fat cells, not useful in TMA cancer-oriented experiments.