Skip to main content
Figure 2 | BMC Bioinformatics

Figure 2

From: Pash 3.0: A versatile software package for read mapping and integrative analysis of genomic and epigenomic variation using massively parallel DNA sequencing

Figure 2

Mapping bisulfite-treated reads. A. During the bisulfite treatment, methylated cytosine bases C's are preserved as C's, but unmethylated cytosine bases are converted into uracil and then, upon DNA amplification, into thymine (T's). B. Ts in the reads can correspond to either T's in the reference or unmethylated C's in the reference. For each sequenced k-mer, we explore the space of possible k-mers by converting the Ts into either Cs or Ts. The k-mers are hashed in the read hash table, after which regular mapping is performed.

Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us