From: Speeding up tandem mass spectrometry-based database searching by longest common prefix
 | Instrument | LTQ |
---|---|---|
 | Spectra | 107666 spectra extracted from ten raw files |
Exp1 | Database | 18 purified proteins with the Uniprot/SwissProt protein sequence database(1020188 protein sequences, target + reversed), total around 460 MB |
 | Digestion way | Site-specific digestion |
 | Tolerance | Precursor: +/- 3Da; Fragment: +/- 0.5 Da |
 | Modifications | Fixed: Carbamidomethylation (C) Variable: Oxidation (M) |
 | Instrument | LTQ-FT or LTQ-Orbitrap |
 | Spectra | 13816 spectra extracted from two raw files |
 | Database | IPI-Mouse protein sequence database(113914 protein sequences, target + reversed), total around 66 MB |
Exp2 | Digestion way | Non-specific digestion |
 | Tolerance | Precursor: +/- 12 ppm; Fragment: +/- 0.5 Da |
 | Modifications | Fixed: Carbamidomethylation (C) Variable: Phosphorylation (S, T, Y) Oxidation (M), |