Figure 7
Results for Ncam1 gene (data from GSE11344). The content of each panel is explained in figure 4. (b) Residuals of the RMA normalization model. It can be observed that for many exons there are residuals that are much larger than expected. This fact indicates the presence of many splicing events. (d) Most of the exons have several probes mapped to them. However, some exons (in the middle close to the 49.34 Mb position) are not represented by any probe. Any splicing event related to these exons cannot be detected. (g) Predicted structure using SPACE of two transcripts of this gene. The first transcript variant 1 is related to the first Ensembl release 51 transcript. The second transcript variant 2 is related to third Ensembl transcript. A few exons are misassigned to these transcripts. (h) Estimated concentrations of each of the two transcripts (variant 1 and 2) in each sample. Samples 1 to 3 correspond to normal tissue where the variant 1 is predominant. Additionally, variant 2 is predominant in samples 4 to 6 that present PTB depletion. These results are in concordance with RT-PCR results.