Skip to main content

Table 2 Contig assembly with FPC and LTC programs

From: LTC: a novel algorithm to improve the efficiency of contig assembly for physical mapping in complex genomes

 

Lib RiceChr1

Lib MaizeChr1

Statistic

FPC

LTC

FPC

LTC

NClonesCtg /NClones (%)

4,293/4,417 (97.2%)

4,231/4,417 (95.7%)

28,899/29,924 (96.6%)

28,994/29,924 (96.9%)

N Ctgs

42

25

257

190

N MeanClonesCtg

102.2

169.2

112.4

152.6

L MeanClonesCtg

1,045 kbp

1,731 kbp

1,128 kbp

1,532 kbp

NChCtg/NCh

183/202 (91%)

22/202 (11%)

946/1,016 (93%)

196/1,016 (19%)

NQNotCh/NQ

105/124 (85%)

106/186 (57%)

955/1,025 (93%)

110/930 (19%)

NCtgsNonLinear/NCtgs

8/42 (19%)

-

23/257 (9%)

-

NCtgCh/NCtgs

22/42 (52%)

0/25

108/257(42%)

0/190

n CtgParts

1.79

1.00

1.67

1.00

NCtgOrdWrong/(NCtgs-NCtgCh)

4/20 (20%)

1/25 (4%)

47/149 (32%)

4/190 (2%)

  1. Here NClonesCtg is the total number of clones in contigs with 6 clones and more (others were excluded from the analysis); NCtgs is the number of contigs with 6 clones and more; NMeanClonesCtg is the mean number of clones in the contigs with 6 clones and more; LMeanClonesCtg = LCloneObs NMeanClonesCtg/Covarege is estimated mean length of the contigs with 6 clones and more; NChCtg is the number of chimerical clones in the contigs with 6 clones and more (false negatives in the procedure of Q-clone detection); NQNotCh is the number of non-chimerical clones that were identified as Q-clones (false positives); NQ is the number of clones that were not included into contigs; NCtgsNonLinear is the number of contigs with non-linear topological structure; NCtgCh is the number of contigs containing clones (except chimerical ones) from non-overlapping parts; nCtgParts is the mean number of non-overlapping parts per contig; NCtgOrdWrong is the number of continuous contigs with errors in clone ordering.