Table 3 The ranking of relative thermostability of wild type proteins and their mutated sequences using the scoring function.

Dmeh (GI: 640374)

51

49

1

Dmeh_UMC

51

99

2

Dmeh_UVF

51

99

3

BsCSP (GI: 16077975)

67

53.8

1

BsCSP_mt1

67

69.7

2

BsCSP_mt2

67

83.7

3

PhyA (GI: 464382)

467

55

1

PhyA_mt18

467

62

2

PhyA_mt24

467

62+

3

PTDH (GI: 194552172)

336

39

1

PTDH_12x

336

59.7

2

PTDH_opt14

336

64.4

3

CbADH (GI: 187935035)

351

64.5

1

cbADH_Q100P

351

76

2

β-GUS (GI: 868020)

602

45

1

β-GUS_TR3337

602

65

2

FAOX (GI: 20302586)

372

37

1

FAOX_TE

372

45

2

Shble (GI: 3891709)

121

67.4

1

Shble_HTS

121

85.1

2

EcHPH (GI: 12539)

341

51

1

EcHPH_hph5

341

67

2

PDAO (GI: 129305)

347

45

2

PDAO_F42C

347

55

1