Table 2 Reactions involved in the MEP pathway (in order of occurrence)
From: Simulation of a Petri net-based Model of the Terpenoid Biosynthesis Pathway
Ordera | Reaction | Enzyme | Main productb |
|---|---|---|---|
A | G3P + pyruvate → DXP + CO2 | DXP synthase (EC 2.2.1.7) | DXP |
B | DXP + NADPH + H+ → MEP + NADP+ | deoxyxylulose phosphate reductoisomerase (DXR; EC1.1.1.267) | MEP |
C | MEP + CTP → CPP-ME + diphosphate | MEP cytidyltransferase (EC 2.7.7.60) | CPP-ME |
D | CPP-ME + ATP → pCPP-ME + ADP | CPP-ME kinase (EC 2.7.1.1480) | pCPP-ME |
E | pCPP-ME → CMP + MEcycPP | MEcycPP synthase (EC4.6.1.12) | MEcycPP |
F | MEcycPP + protein diol → HMBPP + H2O + protein disulfide | HMBPP synthase (EC 1.17.4.3) | HMBPP |
G | HMBPP + NADPH + 2H+ → IPP + DMAPP + H2O + NADP+ | HMBPP reductase (EC 1.17.1.2) | IPP dan DMAPP |
H | IPP + DMAPP → GPP | GPP synthase (EC 2.5.1.10 atau EC 2.5.1.1) | GPP |
I | GPP → monoterpenoid | monoterpene synthase | monoterpenes |