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Figure 7 | BMC Bioinformatics

Figure 7

From: Molecular dynamics simulation studies and in vitro site directed mutagenesis of avian beta-defensin Apl_AvBD2

Figure 7

Evaluation of antibacterial activity of wild type and mutant type Apl_AvBD2. (a) SDS-PAGE of purified recombinant proteins (indicated by arrows). The recombinant wild Apl_AvBD2 showed antibacterial activity against the (b) Gram negative bacteria E. coli (NCIM 2685) and (c) Gram positive bacteria Staphylococcus aureus (NCIM 2654) in the standard plate count assay (SPC). The numbers in graphs represent: (1) wild type (2) F7R (3) I11R (4) V24R (5) F7R I11R (6) F7R I11R V24R (7) F7R I11R V24R S38R. Mutant proteins do not show antibacterial activity.

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