Figure 1
From: Towards reliable isoform quantification using RNA-SEQ data
Distribution of read start position as a percentage length for genes with median length of 1200 nucleotides. The red line is a cubic spline fit. (A) Illumina dataset - RNA fragmentation by sonication [10] (dataset 1), (B) Roche 454 dataset - cDNA fragmentation by nebulization [14], and (C) Simulated cDNA fragmentation assuming fragments in the size range of 500-800 nucleotides.