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Table 4 Data Generation Protocols

From: PEPPI: a peptidomic database of human protein isoforms for proteomics experiments

 

cDNA Sequence Length

Open Reading Frame (ORF)

Stop Codon Toleration

EXON_KB

Whole Length

Annotated ORF Only

Not Tolerated

E_E_KB

≤ 240 bp

Annotated ORF Only

Not Tolerated

E_I_TH

≤ 240 bp

All 3 ORFs

In 3’ end segment

I_E_TH

≤ 240 bp

All 3 ORFs

Not Tolerated

E_E_TH

≤ 240 bp

All 3 ORFs

In 3’ end segment

  1. We limited the lengths of the combination transcripts to 240 bp, with a flanking sequence of 120 bp on each side of the splice site.