Figure 2

High sequence diversity of T3SS effectors. At 100% amino acid sequence identity threshold between the 504 experimentally validated effector sequences, as many as 324 clusters were observed. When the % identity was reduced to 90%, tolerating 10% difference between the sequences, the number of clusters dropped significantly to 206. Allowing more differences between the sequences by reducing the identity threshold (even to as low as 10% identity) did not reduce the number of clusters significantly (171 clusters even at 10% identity). This highlights the high level of amino acid difference between T3SS effectors.