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Figure 1 | BMC Bioinformatics

Figure 1

From: Assessment of genetic variation for the LINE-1 retrotransposon from next generation sequence data

Figure 1

Allele specific confirmation of genotypes. Gel images of four validation runs using allele specific PCR. Each lane is labeled with a sample identifier D=NA12878(daughter), F=NA12891(father), M=NA12892(mother), and C=Control. For each locus, the samples were run against two pairs of primers, one that would amplify DNA that contained a LINE1 insertion (L1+), and one that would amplify DNA that did not (L1-). The four insertion sites chosen for validation were a) the intergenic region on chromosome 5 located at position 21243489 on human genome build 36.3, and those insertions within the loci for b)TRDN, c) GLCCI1, and d) PRKCA. See the Methods section for more details regarding the primer sequences and PCR conditions.

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