Figure 3

Eukaryotic ePST Generation Process. a. Options 1 & 2: Map the peptide to the translated genome. b. Option 1: Extend the mapped peptide in the 3' direction to an in-frame stop codon or splice site boundary. Option 2: Extend the mapped peptide in the 3' direction the number of codons selected by the user. c. Option 1: Extend the mapped peptide in the 5' direction to an in-frame stop codon or start codon, or splice site boundary. Option 2: Extend the mapped peptide in the 5' direction the number of codons selected by the user. d. Final ePST. e. Generate translated ePST sequence.