Figure 2

Kinetics of ribosomes on an RNA strand. (A) Example of the kinetics of several ribosomes along an mRNA template that suffered an arrest at nucleotide 1850, from the moment the ribosome binding site is formed to the degradation of the mRNA. The continuous gray region in the bottom left corresponds to the untranscribed sequence of the mRNA. (B) Distribution of time intervals between consecutive translation initiation events. (C) Distribution (grey bars) of time intervals between consecutive translation completion events given the presence of a sequence dependent arrest site at nucleotide 1850. The solid black line shows the distribution of time intervals between consecutive translation completion events without the sequence-dependent arrest site, normalized to the same scale. Data is from 600 000 initiation events.