Take gene TCF20 as an example to demonstrate the procedure and prediction result of NSMAP. (a)The region of gene TCF20 and the detected splicing junctions using Tophat. (b) The constructed exons based on the detected splicing junctions for gene TCF20. The number in the parentheses of the each left label is the number of reads falling on the corresponding exon. (c) NSMAP predicts three isoforms for TCF20 with expression levels in the parentheses. (d) The two annotated isoforms of gene TCF20 in the UCSC reference genome.