Figure 2

Overview of CLOTU. Overview/Workflow of CLOTU for high throughput sequences. The rectangular boxes depict the functionality of the three steps of the pipeline. Texts in italics depict the filenames and respective extension of output file names. Filename coloured in brown are files submitted by the user (SEQUENCES.ZIP , TPA.TXT and METADATA.TXT). Filename all_dataset contains all sequences pooled in together. All files colored in green, are input files for new steps in the pipeline (accepted.fas, cluster_out.fas and blastout.txt). Filenames in violet are files where the statistics of each step are listed, appended, and summarized (stat_log.txt, summary.txt, cluster_info.txt and output_bp.txt). The filename in red is the file containing all rejected sequences (rejected.fas, singletons.fas). The filename in pink contains detailed statistics of each tag and sample (unique and overall abundance) in excel format (matrix_table_1.xls). Another file in pink is the matrix table_2.xls output file that contains the top BLAST hit of each OTU described in matrix_table_1. All output files which contain appended data are marked with *.