NeurphologyJ produces accurate neurite length estimation in images of primary hippocampal neurons. (A) Example image of mouse hippocampal neurons analyzed by NeurphologyJ and NeuriteTracer. Neurite tracings are shown in red and somata in blue in analyzed images. The scale bar represents 50 μm. (B) Twenty hippocampal neuron images manually traced using NeuronJ, or automatically traced using NeurphologyJ and NeuriteTracer were compared. Total neurite length (in pixels) obtained by manual tracing is plotted on the X-axis, and total neurite length obtained by automatic tracing was plotted on the Y-axis. Pearson's correlation coefficients and paired two-tailed Student's t-test are indicated.