NeurphologyJ produces accurate neurite length estimation in images of P19 neurons. (A) Example image of P19 neurons analyzed by NeurphologyJ. Neurite tracings are shown in red and somata in blue in the analyzed image. The scale bar represents 50 μm. (B) Eight images of P19 neurons manually traced using NeuronJ, or automatically traced using NeurphologyJ and NeuriteTracer were compared. Total neurite length (in pixels) obtained by manual tracing is plotted on the X-axis, and total neurite length obtained by automatic tracing was plotted on the Y-axis. Pearson's correlation coefficients and paired two-tailed Student's t-test are indicated.