NeurphologyJ produces reliable neurite quantification in images with different signal intensities. (A) Examples of images with different signal intensity analyzed by NeurphologyJ. Original images are rendered in pseudo-color to visualize signal intensity. Neurite tracings are shown in red and somata in blue in analyzed images. The scale bar represents 50 μm. (B) Eight P19 neuron images with varying signal intensity were manually traced using NeuronJ or automatically traced using NeurphologyJ. The resulting total neurite lengths were compared. Each data point consists of three parameters: the X-axis value represents the neurite length (in pixels) obtained by manual tracing; the Y-axis value represents the neurite length obtained by NeurphologyJ, and the size of each data point is in proportion to the average signal intensity of that image. Pearson's correlation coefficients and paired two-tailed Student's t-test are indicated.