NeurphologyJ produces accurate soma counts. (A) An example of soma quantification on P19 neurons using NeurphologyJ. The merged image shows the NeurphologyJ identified somata in blue and the original single-channel image in gray. The scale bar represents 50 μm. (B) A comparison of soma number using manual counting and NeurphologyJ automatic counting. Ten images of TUJ1 stained P19 neurons were used for this comparison. Soma counts obtained manually were plotted on the X-axis, and soma counts obtained by NeurphologyJ were plotted on the Y-axis. Pearson's correlation coefficients and paired two-tailed Student's t-test are indicated.