Table 2 Comparisons between the two automatic ImageJ-based methods NeurphologyJ and NeuriteTracer
Measurements (Function, speed, and accuracy) | NeuriteTracer | NeurphologyJ |
|---|---|---|
Neurite length | Yes | Yes |
Soma number | Yes | Yes |
Soma size | No | Yes |
Attachment point | No | Yes |
Ending point | No | Yes |
Image for analysis | Pair of images | Single image |
No. of Images in one batch | Limited by memory | Unlimited |
Analysis speed per image | 2.1 sec | 1.7 sec |
Correlation coefficient/p-value* (primary neurons) | 0.97/<0.0001 | 0.97/0.2696 |
Correlation coefficient/p-value* (P19 neurons) | 0.97/0.0025 | 0.99/0.5678 |