Lipid droplet and nucleus segmentation. Low H2Av-GFP intensity gradient in an optical section between a distinct lipid droplet and a nucleus. (a)-(b) 2D segmentation contours using 16-bit θ
= 9131 (a) and θ
= 9574 (b). The lipid droplet locates in the upper left region of the image, intersected by the white profile line. Size of the images (a) and (b): 10.8 μm × 10.8 μm. (c) Greyscale intensity profile plot. The intensity values of the lipid droplet and the nucleus range between 2 - 4 μm and 5 - 10 μm respectively.