Time-lapse mitotic volume change of a nucleus at syncytial blastoderm stage. The volume of a representative syncytial blastoderm nucleus was measured after it was segmented using the proposed GT segmentation method (red), an alternative GT segmentation method (blue), and the MS method (black) using various parameter values such as k = 0 (solid), k = 0.2 (dashed) and k = (-0.2) (dotted). The insets show representative optical slices of the prophase nucleus with the segmentation contours of MS k = 0 (red) and GT (green). The holes of the MS resulted in different volume measurements at 4 and 5 minutes. The nucleus is in prophase (4-5 min), prometaphase (7 min), metaphase (8-9 min), anaphase (10 min) and telophase (11-13 min).