Gebiss: an ImageJ plugin for the specification of ground truth and the performance evaluation of 3D segmentation algorithms

Table 1 Comparison of mutant Drosophila brain volumes

Genotype 1	Genotype 2	p-value	volume difference^a[%]
C155/yw	C155/+; EP2299/+	< 0.001	+ 14.6%
YW	bchs58/bchs58	< 0.001	- 16.9%
YW	bchs58/Df(2L)clot7	< 0.001	- 15.5%
bchs58/bchs58	bchs58/Df(2L)clot7	0.641	NA
C155/+; EP2299/+	bchs58/bchs58	< 0.001	- 10.3%
C155/+; EP2299/+	bchs58/Df(2L)clot7	< 0.01	- 8.8%

Using Gebiss we show that both bchs null mutant combinations of bchs58/Df(2L)clot7 and homozygous bchs58 have a reduction in the brain volumes of third instar larvae, when compared with the background genotype YW brains as a control. ^aThe difference in brain volume of Genotype 2 in comparison with Genotype 1 as the reference control. NA: Non-applicable.

ISSN: 1471-2105