Program | Primer | Sequence | Tm (C) | 5' Location | Length |
---|---|---|---|---|---|
LAVA | F1 | GGAATAGTTTGTAAGACACCTGCCA | 55.82 | 149 | 25 |
 | F2 | ACCAACACCAAAAATCGGT | 50.22 | 103 | 19 |
 | F3 | GCTACAATTGCAGGCGTTT | 51.66 | 83 | 19 |
 | B1 | CAAAAACAAAGCGAACTGCCAAT | 54.18 | 209 | 23 |
 | B2 | TGGCATTATTACTTGCCATCA | 50.38 | 260 | 21 |
 | B3 | TTGATGTCGAAAACACTGGAA | 50.63 | 300 | 21 |
PrimerExplorer | F1 | TGTTGGAATAGTTTGTAAGACACCT | 53.22 | 145 | 25 |
 | F2 | TTACCAACACCAAAAATCGG | 48.91 | 101 | 21 |
 | F3 | GCTACAATTGCAGGCGTT | 50.80 | 83 | 18 |
 | B1 | CAAAAACAAAGCGAACTGCCAATA | 53.99 | 209 | 24 |
 | B2 | GCATTATTACTTGCCATCATTG | 48.63 | 258 | 22 |
 | B3 | TGTCGAAAACACTGGAACAT | 50.11 | 296 | 20 |