Supplementary Data Fields & Prioritisation Features. Schematics summarizing the features used to prioritise the resulting putative PPIs. For each PPI, a number of metadata fields are collected during the three main steps of the algorithm. Two metrics can optionally be computed: an Interolog Prioritisation Index (IPX) and a PPI Conservation Score (PCS) (Additional File 2 - Definitions). A. Overall view showing the contributing data fields. B-E. Sample prioritisation features. B: Phylogenetic distances (according to TreeBeST). For each of the two orthologous pairs, a node-to-node distance (nnD
) and two distances from the First Shared Ancestor are computed. C: experimental interaction observed in multiple taxa - a component of the IPX is proportional to the number of reference genomes contributing to a putative PPI evidence. D: experimental interaction reconfirmed through multiple detection methods - a component of the IPX is proportional to the number of detection methods used to obtain experimental PPI evidence in the reference genome. E: PPI Conservation Score. The conservation score depends on (1) the density of the most-connected γ -clique that includes x, y and their mutual interactors and (2) the number of edges of the γ clique.