Figure 4

Five complexes implicated in HIV pathogenesis by analysis with CORUM. (A) Network analysis of RNAi datasets. Gray nodes are subunits present in the complex according to the CORUM database. Colored subunits (nodes) were reported in one or more of the genetic screens. Based on the hypergeometric distribution, we find significantly more subunits of the proteasome (p = 4.2 × 10^-9), Mediator (p = 1.1 × 10^-9), and the exosome (p = 2.1 × 10^-3) than expected. Subunits of ESCRT III and CCT complexes are not significantly enriched. The table shows the number of complexes and subunits identified by two, three or four RNAi screens. As with genetic screens, there is greater overlap between datasets when analyzed in terms of subunits of complexes as opposed to isolated proteins. (B) Network analysis of proteomic profiling datasets. The same complexes are shown as in panel A, with subunits highlighted as they occur in different datasets. Mediator and exosome complexes are not covered more than expected, but significantly more subunits than expected are found for ESCRT III (p = 8.4 × 10^-3) and CCT complexes (p = 2.0 × 10^-7). The proteasome is the only complex where more subunits than expected are identified by both genetic and proteomic profiling screens (p = 7.0 × 10^-23).