Figure 5

Correction of errors in quantitative data. a) Confocal image of Drosophila embryo stained for the expression of hb gene and scanned with offset -40% and gain 705V. The image belongs to S3. Quantitative data are extracted from the area outlined in the image. b) 1D expression pattern of hb gene extracted from the image (black circles) and corrected to eliminate the saturation effect (white circles). Differences between corrected and observed quantitative gene expression data are shown by crosses. c) Quantitative data on ftz gene expression extracted from the image taken by the same microscope as sample S2 with offset -2% and gain 1100V (the image is not shown).