Offset\gain,V | 500 | 550 | 600 | 650 | 700 | 750 | 800 | 850 | 900 | 1000 |
---|
0 | I(2) | I | I(5) | I | I(2),II(2),III | II | I,II(5),III(3) | II | I,II(3),III | II(3) |
-1%(10) | I(2) | I | I | I | I(2),II(2),III | II | I,II(2),III | II | I,II(2),III | - |
-2%(20) | I(2) | I | I | I | I(2),II(2),III | II | I,II(3),III | II | I,II(3),III | II |
-4%(40) | - | I | - | - | - | - | II | - | II | II |
- Combinations of PMT gain and offset used for the acquisition of 76 images. The gain values in parentheses are corrected to combine all the samples (see text). Offset values in parentheses are measured in intensity units subtracted from the image. Images are obtained in 3 microscope channels using different lasers (I - 488 nm, II - 543 nm; III - 633 nm). The table entries are the channel numbers in which images at the corresponding combination of PMT parameters are obtained; if more than one image is obtained the number of images is given in parentheses.