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Table 1 Learning sample S1

From: A regression system for estimation of errors introduced by confocal imaging into gene expression data in situ

Offset\gain,V

500

550

600

650

700

750

800

850

900

1000

0

I(2)

I

I(5)

I

I(2),II(2),III

II

I,II(5),III(3)

II

I,II(3),III

II(3)

-1%(10)

I(2)

I

I

I

I(2),II(2),III

II

I,II(2),III

II

I,II(2),III

-

-2%(20)

I(2)

I

I

I

I(2),II(2),III

II

I,II(3),III

II

I,II(3),III

II

-4%(40)

-

I

-

-

-

-

II

-

II

II

  1. Combinations of PMT gain and offset used for the acquisition of 76 images. The gain values in parentheses are corrected to combine all the samples (see text). Offset values in parentheses are measured in intensity units subtracted from the image. Images are obtained in 3 microscope channels using different lasers (I - 488 nm, II - 543 nm; III - 633 nm). The table entries are the channel numbers in which images at the corresponding combination of PMT parameters are obtained; if more than one image is obtained the number of images is given in parentheses.