Sensitivity, Specificity and Accuracy in Comparison. Sensitivity, specificity and accuracy for our approach without and with secondary antibody binding removal in comparison to the approaches of  and . Both, the high (3 ng/ml) and the low (1 ng/ml) spike-in antibody concentration slides were evaluated for both print batches (left and right). 95% bootstrap confidence intervals were computed based on 1000 times resampled peptide intensities and are shown by dashed lines. For all approaches, the specificity remains rather constant when reducing the spike-in antibody concentration, while we see a general decline in sensitivity and accuracy. For the approaches of  and  the decline in accuracy is rather steep, our approach shows still good accuracy above 0.8 for the low antibody concentration.