Figure 3

Sensitivity, Specificity and Accuracy in Comparison. Sensitivity, specificity and accuracy for our approach without and with secondary antibody binding removal in comparison to the approaches of [2] and [17]. Both, the high (3 ng/ml) and the low (1 ng/ml) spike-in antibody concentration slides were evaluated for both print batches (left and right). 95% bootstrap confidence intervals were computed based on 1000 times resampled peptide intensities and are shown by dashed lines. For all approaches, the specificity remains rather constant when reducing the spike-in antibody concentration, while we see a general decline in sensitivity and accuracy. For the approaches of [2] and [17] the decline in accuracy is rather steep, our approach shows still good accuracy above 0.8 for the low antibody concentration.