Execution profile of an analysis with CloVR-Microbe. CloVR-Microbe was used to perform whole-genome shotgun (WGS) assembly and annotation on 500,000 3 kbp paired-end sequence reads generated with the 454 Titanium FLX platform from a Escherichia coli whole-genome shotgun library (unpublished data). The local VM client first started a remote (master) VM instance on the cloud. The input sequencing reads (676 MB, compressed SFF file) were copied to this instance and assembled on a single c1.xlarge VM instance, using no more than no more than two out of the eight available CPUs. Then, prior to the genome annotation, which involves several parallelizable search steps, 15 additional CloVR VM instances were allocated to improve processing throughput. A configurable parameter limits the number of instances that are added. Idle instances are subsequently terminated automatically upon job completion on an hourly timer.